Evaluation of laccases and melanization in clinical and environmental Cryptococcus neoformans samples by non-denaturing PAGE
نویسندگان
چکیده
منابع مشابه
Vesicle-associated melanization in Cryptococcus neoformans.
Recently, several pathogenic fungi were shown to produce extracellular vesicles that contain various components associated with virulence. In the human pathogenic fungus Cryptococcus neoformans, these components included laccase, an enzyme that catalyses melanin synthesis. Spherical melanin granules have been observed in the cell wall of C. neoformans. Given that melanin granules have dimension...
متن کاملGenetic study of oxygen resistance and melanization in Cryptococcus neoformans.
Genetic analysis of oxygen-sensitive mutants of Cryptococcus neoformans revealed two loci (oxy1 and oxy2) linking hyperoxia sensitivity to production of melanin, a known virulence factor. Hyperoxia-sensitive strain 562 (oxy1 oxy2) is albino and avirulent. oxy2-defective strains lacking the oxy1 defect are melanin deficient but show normal hyperoxia resistance. Mutants defective at three additio...
متن کاملCatecholamine uptake, melanization, and oxygen toxicity in Cryptococcus neoformans.
Oxygen sensitivity mutations of Cryptococcus neoformans were mapped to three genetic loci. Three oxygen-sensitive mutants had mutations that appeared allelic and exhibited albinism tightly linked to oxygen sensitivity; these three and a fourth exhibited defects in catechol uptake and catechol oxidation to melanin. Catecholamine metabolism appears to protect C. neoformans from oxidants.
متن کاملLong-term survival of Cryptococcus neoformans and Cryptococcus gattii in stored environmental samples from Colombia.
BACKGROUND Both Cryptococcus neoformans and Cryptococcus gattii have been isolated from a variety of environmental sources in Colombia. AIM To determine the viability of C. neoformans/C. gattii isolates in stored soil samples, filtrates and bird droppings from which these yeasts were previously recovered. METHODS A total of 964 samples collected between 2003 and 2009, and kept at room tempe...
متن کاملChromosome length polymorphism in Cryptococcus neoformans clinical and environmental isolates.
A protocol for intact DNA preparation from the basidiomycetous yeast Cryptococcus neoformans has been developed and applied to karyotyping C. neoformans isolates displaying different degrees of capsule formation. A total of 46 strains have been analyzed: 23 (50%) isolated from environmental samples (pigeon droppings), all of them belonging to C. neoformans var. neoformans; and 23 (50%) from cli...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2009
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.007716-0